|
|
| This project is collaborated between The Forsyth Institute (TFI) and The Institute for Genomic Research (TIGR), and is funded by National Institute of Dental and Craniofacial Research (NIDCR) |
|
|||||||||||||||||||||||||
Number of rRNA operonsAn assessment of the number and location of large multicopy regions in the genome, such as rrna operons, will help prevent ambiguities in the genome sequence assembly phase. We have preliminary data indicating that Porphyromonas gingivalis W83, and other strains tested, possesses four rrna operons. Ribotyping was performed on a large number of reference and clinical strains using Kpn I, Pst I, BamH I, and Bgl II restriction endonucleases. In all cases, four bands were seen in Southern blots using a 1000 base fragment of the 16S rRNA gene as a probe (PCR amplified using 16S rRNA 340-357 forward and 1369-1387 reverse primers). CeuI, a restriction endonuclease which recognizes a conserved 19 base region of the 23S rRNA gene, cuts genomes once for each rRNA cistron. CeuI- digestion of P. gingivalis chromosomal DNA showed 4 bands by PFGE, consistent with the results from ribotyping. |
||||
|
This page is created and maintained by Drs. Margaret Duncan, Floyd Dewhirst, and Tsute Chen, Department of Molecular Genetics, The Forsyth Institute . Last modified on 02/20/2002 Copyright 2000, 2001, 2002 by The Forsyth Institute |
