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| This project is collaborated between The Forsyth Institute (TFI) and The Institute for Genomic Research (TIGR), and is funded by National Institute of Dental and Craniofacial Research (NIDCR) |
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Size of the P. gingivalis genomeThe genome size of P. gingivalis strain W83 was determined by digestion of genomic DNA with Xba I (TCTAGA) and Spe I (ACTAGT) which previously had been found to yield a small number of large restriction fragments separable by pulsed field electrophoresis (Fraser and Duncan, 1992). Pulsed field gel electrophoresis of chromosome digests were carried out using a Bio-Rad CHEF DRIII with run conditions determined by the CHEF Mapper. Gels were run under a variety of conditions to resolve poorly separated bands. Fragment sizes were estimated by comparison with the mobility of lambda concatamers and low molecular weight markers. From the Xba I digests the genome size was calculated to be 2,176 kb, and 2,250 kb from the Spe I digests.
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This page is created and maintained by Drs. Margaret Duncan, Floyd Dewhirst, and Tsute Chen, Department of Molecular Genetics, The Forsyth Institute . Last modified on 02/20/2002 Copyright 2000, 2001, 2002 by The Forsyth Institute |
PFGE of Xba I- and Spe I-digested DNA from P. gingivalis
strains. Lane 1, Lambda concatamers: 2, strain 381; 3, 33277; 4, A5B3;
5, A7436; 6, W83; 7, HG405; 8, ITA-1; 9, W50.